It was the second air force accident in less than a week.
A ministry spokesman said the F-18 jet crashed at the Torrejon de Ardoz base outside Madrid during takeoff.
Television images showed a massive cloud of smoke rising from the base area.
Mr Pérez Serrano joined the air force in 2009 and had logged more than 700 flight hours, Spanish media reports.
However, the Spanish Ministry of Defence has not confirmed the reason for the crash.
“The pilot of the airplane has died as a outcome of the accident, which happened during take-off”, the Spanish defence ministry tweeted.
The cause of crash of plane was announced that investigation into accident was not yet known.
It was returning from Madrid after taking part in celebrations for Spain’s National Day. The ministry confirmed that the pilot has died.
The 34-year-old Eurofighter pilot was also killed on approach to landing at the Albacete military base, some 300 kilometers (186 miles) southeast of Madrid.
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This is happening more frequently and there should be an investigation
I now of the problems and figuring out what the problem is, the hostin
I don't know if it's just me or if perhaps everybody else encountering
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